Effect of tetracycline derivatives and some cations on the activity of anhydrotetracycline oxygenase

• Published in: Biotechnology letters Vol. 5; no. 8; pp. 537 - 542
• Main Authors: BEHAL, V, NEUZIL, J, HOSTALEK, Z
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.1983
• Subjects: Analytical, structural and metabolic biochemistry; anhydrotetracycline oxygenase; Biological and medical sciences; Biotechnology; calcium; Enzyme engineering; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Improved methods for extraction and purification of enzymes; ions; magnesium; metals; Methods. Procedures. Technologies; Oxidoreductases; pharmacology; Streptomyces; tetracycline; Purification; Actinomycetaceae; Cations; Catalyst activity; Gel permeation chromatography; Ion exchange chromatography; Streptomyces aureofaciens
• DOI: 10.1007/BF01184945
• ISSN: 0141-5492

Anhydrotetracycline oxygenase was partially purified on a Sephadex G-25 column and DEAE-cellulose. The purified enzyme was inhibited by tetracycline derivatives added to the reaction mixture. Certain concentrations of calcium and magnesium ions stimulated the enzymic activity; further increase of the concentration resulted in a sharp decrease of the enzymic activity. The enzyme activity was also stimulated by Cu super(2+), Ni super(2+), Co super(2+), Fe super(3+) and Mn super(2+) present in suitable concentrations in the reaction mixture.