Effect of tetracycline derivatives and some cations on the activity of anhydrotetracycline oxygenase

Anhydrotetracycline oxygenase was partially purified on a Sephadex G-25 column and DEAE-cellulose. The purified enzyme was inhibited by tetracycline derivatives added to the reaction mixture. Certain concentrations of calcium and magnesium ions stimulated the enzymic activity; further increase of th...

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Bibliographic Details
Published inBiotechnology letters Vol. 5; no. 8; pp. 537 - 542
Main Authors BEHAL, V, NEUZIL, J, HOSTALEK, Z
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.01.1983
Subjects
Analytical, structural and metabolic biochemistry
anhydrotetracycline oxygenase
Biological and medical sciences
Biotechnology
calcium
Enzyme engineering
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Improved methods for extraction and purification of enzymes
ions
magnesium
metals
Methods. Procedures. Technologies
Oxidoreductases
pharmacology
Streptomyces
tetracycline
Purification
Actinomycetaceae
Cations
Catalyst activity
Gel permeation chromatography
Ion exchange chromatography
Streptomyces aureofaciens
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Summary:Anhydrotetracycline oxygenase was partially purified on a Sephadex G-25 column and DEAE-cellulose. The purified enzyme was inhibited by tetracycline derivatives added to the reaction mixture. Certain concentrations of calcium and magnesium ions stimulated the enzymic activity; further increase of the concentration resulted in a sharp decrease of the enzymic activity. The enzyme activity was also stimulated by Cu super(2+), Ni super(2+), Co super(2+), Fe super(3+) and Mn super(2+) present in suitable concentrations in the reaction mixture.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0141-5492
1573-6776
DOI:10.1007/BF01184945