A structured growth model for Cynara cardunculus cell suspension

In this study a model was developed to describe the growth of Cynara cardunculus L. suspended cells as a function of the availability of two substrates, sucrose as the carbon and energy source and phosphate. It was assumed that the maintenance energy need was fulfilled by the consumption of extracel...

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Published inBioprocess engineering (Berlin, West) Vol. 23; no. 2; pp. 199 - 203
Main Authors PIRES CABRAL, P. M. N. A. C, LIMA COSTA, M. E, CABRAL, J. M. S
Format Journal Article
LanguageEnglish
Published Heidelberg Springer 18.08.2000
Berlin
Subjects
Biological and medical sciences
Biotechnology
Cynara cardunculus
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
phosphate
Plant cells and fungal cells
Culture medium
Cell proliferation
Phosphates
Cell culture
Vegetals
Sucrose
Compositae
Suspension culture
Vegetable crop
Cynara cardunculus
Dicotyledones
Angiospermae
Spermatophyta
Mathematical model
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Summary:In this study a model was developed to describe the growth of Cynara cardunculus L. suspended cells as a function of the availability of two substrates, sucrose as the carbon and energy source and phosphate. It was assumed that the maintenance energy need was fulfilled by the consumption of extracellular carbohydrates, in non-limiting conditions, or by the consumption of structural biomass when sucrose is depleted. A production of secondary metabolites was also assumed. This model was developed based on a structured model previously described by Van Gulik et al. (1993). The model was applied to the experimental results of C. cardunculus suspended cells grown in a Gamborg B sub(5) medium supplemented with 2% sucrose, using a non-linear regression program.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0178-515X
1432-0797
DOI:10.1007/PL00009122