Nafion membrane incorporated with silver nanoparticles as optical test strip for dissolved hydrogen peroxide: Preparation, deployment and the mechanism of action

• Published in: Sensors and actuators. B, Chemical Vol. 255; pp. 605 - 615
• Main Authors: Naik, Apurva N., Patra, Sabyasachi, Kanekar, Avinash S., Sen, Debasis, Ramagiri, Shobha V., Bellare, Jayesh R., Mazumder, Subhasish, Goswami, Asok
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.02.2018; Elsevier Science Ltd
• Subjects: Ascorbic acid; Biosensors; Chemical synthesis; Gas absorption; Hydrogen peroxide; Nafion-117; Nanocomposites; Nanoparticles; Oxidative sawing model; Radioactive tracers; Reduction; Sawing; Silver; Silver nanoparticles; Small angle X ray scattering; Strip; Hydrogen peroxide; Ascorbic acid; Oxidative sawing model; Silver nanoparticles; Nafion-117
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2017.08.103

[Display omitted] •Nafion membrane incorporated with AgNPs is used as a test strip for H2O2.•AgNPs with good optical quality are synthesised by novel ascorbic acid reduction.•The strip is highly selective for H2O2 with excellent percentage recovery.•The strip is successfully employed for quantitative detection of H2O2 in milk.•The mechanism of detection is explored and an Oxidative Sawing Model is proposed. We report the preparation of an optical test strip for quantitative assay of hydrogen peroxide (H2O2) in aqueous solution. Silver nanoparticles(AgNPs) with good optical quality are synthesized by in-situ reduction of silver ions in Nafion-117 membrane previously absorbed with ascorbate ion (HA−). The nanocomposite membrane exhibits a narrow Localized Surface Plasmon Resonance (LSPR) band at 413nm. The extent of decrease in intensity of the LSPR band in presence of H2O2 solution gives quantitative estimate of H2O2 concentration. The detector has been found to show a good analytical response towards H2O2 detection at pH 7 over a wide concentration range. The detection limit has been calculated to be 2.6×10−8molL−1, which is lower than the conventional enzyme based biosensors. The present test strip has been succesfully employed for the quantitative assay of H2O2 in milk. The mechanism of change in LSPR in presence of H2O2 (1.0×10−3molL−1) has been explored for the first time using time resolved Small-Angle X-ray Scattering and radiotracer techniques. The results show that at this concentration of H2O2, its sharp oxidative sawing action is responsible for cutting off the nanoparticles into smaller fragments resulting in rapid reduction of absorption crossection.