Redox-related peroxidative responses evoked by methyl-jasmonate in axenically cultured aeroponic sunflower (Helianthus annuus L.) seedling roots

• Published in: Protoplasma Vol. 221; no. 1-2; pp. 79 - 91
• Main Authors: Garrido, I, Espinosa, F, Córdoba-Pedregosa, M C, González-Reyes, J A, Alvarez-Tinaut, M C
• Format: Journal Article
• Language: English
• Published: Austria Springer Nature B.V 01.05.2003
• Subjects: Acetates - pharmacology; Cyclopentanes - pharmacology; Enzymes; Extracellular Space - metabolism; Ferricyanides - metabolism; Helianthus - growth & development; Helianthus - metabolism; Hydrogen Peroxide - metabolism; Hypotheses; NAD - metabolism; Oxidation-Reduction; Oxygen Consumption; Oxylipins; Plant Roots - drug effects; Plant Roots - metabolism; Potassium - metabolism; Proteins; Protons; Seedlings - drug effects; Seedlings - growth & development; Seedlings - metabolism
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-002-0073-0

Methyl-jasmonate (MeJA) has been proposed to be involved in the evocation of defense reactions, as the oxidative burst in plants, substituting the elicitors or enhancing their effect. 48 h dark- and sterilely cultured (axenic) aeroponic sunflower seedling roots excised and treated with different concentrations of MeJA showed a strong and quick depression of the H(+) efflux rate, 1.80 microM MeJA totally stopping it for approximately 90 min and then reinitiating it again at a lower rate than controls. These results were wholly similar to those obtained with nonsterilely cultured roots and have been interpreted as mainly based on H(+) consumption for O(2)(*-) dismutation to H(2)O(2). Also K(+) influx was strongly depressed by MeJA, even transitorily reverting to K(+) efflux. These results were consistent with those associated to the oxidative burst in plants. MeJA induced massive H(2)O(2) accumulation in the middle lamella and intercellular spaces of both the root cap cells and the inside tissues of the roots. The native acidic extracellular peroxidase activity of the intact (nonexcised) seedling roots showed a sudden enhancement (by about 52%) after 5 min of MeJA addition, maintained for approximately 15 min and then decaying again to control rates. O(2) uptake by roots gave similar results. These and other results for additions of H(2)O(2) or horseradish peroxidase, diphenylene iodonium, and sodium diethyldithiocarbamate trihydrate to the reaction mixture with roots were all consistent with the hypothesis that MeJA induced an oxidative burst, with the generation of H(2)O(2) being necessary for peroxidase activity. Results with peroxidase activity of the apoplastic fluid were in accordance with those of the whole root. Finally, MeJA enhanced NADH oxidation and inhibited hexacyanoferrate(III) reduction by axenic roots, and diphenylene iodonium cancelled out these effects. Redox activities by CN(-)- preincubated roots were also studied. All these results are consistent with the hypothesis that MeJA enhanced the NAD(P)H oxidase of a redox chain linked to the oxidative burst, so enhancing the generation of O(2)(*-) and H(2)O(2), O(2) uptake, and peroxidase activity by roots.