Comparative Genome analysis of the Genus Curvibacter and the Description of Curvibacter microcysteis sp. nov. and Curvibacter cyanobacteriorum sp. nov., Isolated from Fresh Water during the Cyanobacterial Bloom Period

• Published in: Journal of microbiology and biotechnology Vol. 33; no. 11; pp. 1428 - 1436
• Main Authors: Le, Ve Van, Ko, So-Ra, Kang, Mingyeong, Jeong, Seonah, Oh, Hee-Mock, Ahn, Chi-Yong
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.11.2023
• Subjects: Bacterial Typing Techniques; Cyanobacteria - genetics; DNA; DNA, Bacterial - genetics; Fatty Acids - analysis; Fresh Water; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; 생물학; cyanobacterial bloom; Microcystis; Curvibacter
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2306.06017

The three Gram-negative, catalase- and oxidase-positive bacterial strains RS43 , HBC28, and HBC61 , were isolated from fresh water and subjected to a polyphasic study. Comparison of 16S rRNA gene sequence initially indicated that strains RS43 , HBC28, and HBC61 were closely related to species of genus and shared the highest sequence similarity of 98.14%, 98.21%, and 98.76%, respectively, with 7-1 . Phylogenetic analysis based on genome sequences placed all strains within the genus . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the three strains and related type strains supported their recognition as two novel genospecies in the genus . Comparative genomic analysis revealed that the genus possessed an open pangenome. Based on KEGG BlastKOALA analyses, species have the potential to metabolize benzoate, phenylacetate, catechol, and salicylate, indicating their potential use in the elimination of these compounds from the water systems. The results of polyphasic characterization indicated that strain RS43 and HBC61 represent two novel species, for which the name sp. nov. (type strain RS43 =KCTC 92793 =LMG 32714 ) and sp. nov. (type strain HBC61 =KCTC 92794 =LMG 32713 ) are proposed.