Sperm losses during deep-freeze processing of bull semen

Ejaculates collected from 12 bulls were split and processed either by normal deep-freeze procedure including cooling to 4°C prior to glycerolisation and equilibration or by a modified procedure where glycerolisation and equilibration were carried out at ambient temperature (18°C). Semen from both tr...

Full description

Saved in:
Bibliographic Details
Published inTheriogenology Vol. 21; no. 6; pp. 1001 - 1004
Main Authors Morris, G.R., Burton, L.J., Pitt, C.J.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1984
Subjects
Bovine spermatozoa
cold glassware
deep-freeze processing
losses
deep-freeze processing
Bovine spermatozoa
losses
cold glassware
Online AccessGet full text

Cover

More Information
Summary:Ejaculates collected from 12 bulls were split and processed either by normal deep-freeze procedure including cooling to 4°C prior to glycerolisation and equilibration or by a modified procedure where glycerolisation and equilibration were carried out at ambient temperature (18°C). Semen from both treatments was packed into 0.25-ml French straws and frozen on horizontal racks in liquid nitrogen vapour. The concentration of spermatozoa recovered from straws from each treatment had a mean difference of 12.6 ± 2.7 million per millilitre (p<0.001). The 11% lower concentration of spermatozoa in the normally processed frozen semen was associated with sperm adhering to cold glassware.
Bibliography:L10
8505447
ISSN:0093-691X
1879-3231
DOI:10.1016/0093-691X(84)90394-7