Developing a styrylpyridinium-based fluorescent probe with excellent sensitivity for visualizing basal H2S levels in mitochondria

[Display omitted] •MNOP-H2S is highly sensitive to sense H2S with a detection limit of 29 nM.•MNOP-H2S can monitor mitochondrial basal H2S levels.•MNOP-H2S is applied for distinguishing cancer cells from normal cells.•MNOP-H2S is applied to verify role of H2S in endothelial cell migration. Hydrogen...

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Bibliographic Details
Published inSensors and actuators. B, Chemical Vol. 327; p. 128937
Main Authors Ma, Bin, Tian, Di-Hua, Yan, Shuai, Li, Xiao-Chun, Dai, Fang, Zhou, Bo
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 15.01.2021
Elsevier Science Ltd
Subjects
Cancer
Endothelial cells
Fluorescent indicators
Fluorescent probe
Growth factors
Hydrogen sulfide
Mitochondria
Selectivity
Sensitivity
Styrylpyridinium
Zebrafish
Hydrogen sulfide
Styrylpyridinium
Mitochondria
Fluorescent probe
Cancer
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Summary:[Display omitted] •MNOP-H2S is highly sensitive to sense H2S with a detection limit of 29 nM.•MNOP-H2S can monitor mitochondrial basal H2S levels.•MNOP-H2S is applied for distinguishing cancer cells from normal cells.•MNOP-H2S is applied to verify role of H2S in endothelial cell migration. Hydrogen sulfide (H2S) is recognized as a critical gaseous signaling molecule involved in multiple physiological and pathological processes. The important pathophysiological roles of H2S have spurred intense interest in developing fluorescent probes for imaging of H2S. Using a piperazidine-bridged styrylpyridinium as the push-pull fluorophore and a 7-nitro-1,2,3-benzoxadiazole moiety as the response group, herein we developed a mitochondria-targeted fluorescent probe MNOP-H2S, which exhibited remarkable fluorescence turn-on (>130 fold), large Stokes shift (190 nm), excellent sensitivity and selectivity. Importantly, the probe was characterized by an ultralow detection limit (29 nM), thereby being successfully applied to monitor mitochondrial basal H2S levels in various types of cells and zebrafish, to distinguish cancer cells from normal cells (even from each other) and to reveal that the endogenously produced H2S in mitochondria is a key mediator for the endothelial cell migration stimulated by vascular endothelial growth factor.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.128937