Preparation and Performance Evaluation of a Zinc Oxide-Graphene Oxideloaded Chitosan-Based Thermosensitive Gel

• Published in: Journal of microbiology and biotechnology Vol. 34; no. 6; pp. 1229 - 1238
• Main Authors: Huang, Hao, Han, Rui, Huang, Ping-Ping, Qiao, Chuan-Yue, Bian, Shuang, Xiao, Han, Ma, Lei
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.06.2024
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Biofilms - drug effects; Cell Line; Chitosan - chemistry; Chitosan - pharmacology; Glycerophosphates - chemistry; Graphite - chemistry; Hydrogels - chemistry; Hydrogen-Ion Concentration; Mice; Microbial Sensitivity Tests; Nanoparticles - chemistry; Porphyromonas gingivalis - drug effects; Temperature; Zinc Oxide - chemistry; Zinc Oxide - pharmacology; 생물학; chitosan; graphene oxide; antibacterial; nano zinc oxide; Thermosensitive hydrogel
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2402.02055

This study aimed to develop and assess a chitosan biomedical antibacterial gel ZincOxide-GrapheneOxide/Chitosan/β-Glycerophosphate (ZnO-GO/CS/β-GP) loaded with nano-zinc oxide (ZnO) and graphene oxide (GO), known for its potent antibacterial properties, biocompatibility, and sustained drug release. ZnO nanoparticles (ZnO-NPs) were modified and integrated with GO sheets to create 1% and 3% ZnO-GO/CS/β-GP thermo-sensitive hydrogels based on ZnO-GO to Chitosan (CS) mass ratio. Gelation time, pH, structural changes, and microscopic morphology were evaluated. The hydrogel's antibacterial efficacy against Porphyromonas gingivalis, biofilm biomass, and metabolic activity was examined alongside its impact (MC3T3-e1). The findings of this study revealed that both hydrogel formulations exhibited temperature sensitivity, maintaining a neutral pH. The ZnO-GO/CS/β-GP formulation effectively inhibited P. gingivalis bacterial activity and biofilm formation, with a 3% ZnO-GO/CS/β-GP antibacterial rate approaching 100%. MC3T3-e1 cells displayed good biocompatibility when cultured in the hydrogel extract.The ZnO-GO/CS/β-GP thermo-sensitive hydrogel demonstrates favorable physical and chemical properties, effectively preventing biofilm formation. It exhibits promising biocompatibility, suggesting its potential as an adjuvant therapy for managing and preventing peri-implantitis, subject to further clinical investigations.