Cross talk between kinin and angiotensin II receptors in mouse abdominal aorta

• Published in: Biological chemistry Vol. 390; no. 9; pp. 907 - 913
• Main Authors: Rodrigues, Eliete S., Martin, Renan P., Felipe, Sandra A., Bader, Michael, Oliveira, Suzana M., Shimuta, Suma I.
• Format: Journal Article
• Language: English
• Published: Germany Walter de Gruyter 01.09.2009
• Subjects: angiotensin II; Angiotensin II - pharmacology; Animals; Aorta, Abdominal - drug effects; Aorta, Abdominal - metabolism; bradykinin; Bradykinin - pharmacology; cross talk; Imidazoles - antagonists & inhibitors; kinin receptor-deficient mice; Kinins - pharmacology; Mice; Mice, Inbred BALB C; Mice, Transgenic; NG-Nitroarginine Methyl Ester - pharmacology; Polymerase Chain Reaction; Pyridines - antagonists & inhibitors; Receptor, Bradykinin B1 - genetics; Receptor, Bradykinin B1 - metabolism; Receptor, Bradykinin B2 - genetics; Receptor, Bradykinin B2 - metabolism; Receptors, Angiotensin - genetics; Receptors, Angiotensin - metabolism; Vasodilator Agents - pharmacology
• ISSN: 1431-6730; 1437-4315
• DOI: 10.1515/BC.2009.081

Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with genetic disruption of B1 or B2 and both receptors. Responsiveness of abdominal aortic rings to BK and AngII as well as the receptor gene expression of both peptides were analysed. Although no specific phenotype was displayed in the normotensive and healthy mice lacking the kinin receptors, a decreased expression level of the remaining kinin receptor mRNA was observed. AT1 receptor mRNA level was also reduced, indicating that kinin receptors regulate AngII receptors. Downregulation of the receptors was well correlated with reduction in the reactivity of both agonists to induce contraction of aortic rings, but other signal regulations must be sought in these transgenic mice. We conclude that cross talk between kinin and AngII receptors occurs in mouse abdominal aorta and that both peptides may regulate the initiation and progression of important pathophysiological processes, such as hypertension and inflammation.