A 2′,4′-Bridged Nucleic Acid Containing 2-Pyridone as a Nucleobase: Efficient Recognition of a C⋅G Interruption by Triplex Formation with a Pyrimidine Motif

Significantly enhanced binding affinity to C⋅G base pairs without loss of sequence selectivity is achieved by using a nucleotide containing a 2‐pyridone and a 2′‐O,4′‐C‐methylene‐bridged nucleic acid analogue (PB, see picture). The degree of stabilization of the triplex formed enables C⋅G interrupti...

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Published inAngewandte Chemie (International ed.) Vol. 40; no. 11; pp. 2079 - 2081
Main Authors Obika, Satoshi, Hari, Yoshiyuki, Sekiguchi, Mitsuaki, Imanishi, Takeshi
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag GmbH 01.06.2001
WILEY‐VCH Verlag GmbH
Subjects
DNA recognition
nucleic acids
nucleobases
oligonucleotides
DNA recognition
nucleic acids
oligonucleotides
nucleobases
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Summary:Significantly enhanced binding affinity to C⋅G base pairs without loss of sequence selectivity is achieved by using a nucleotide containing a 2‐pyridone and a 2′‐O,4′‐C‐methylene‐bridged nucleic acid analogue (PB, see picture). The degree of stabilization of the triplex formed enables C⋅G interruptions in a homopurine⋅homopyrimidine double‐stranded DNA to be detected.
Bibliography:ArticleID:ANIE2079
ark:/67375/WNG-DVP1S16D-5
istex:B73D18E763561B79A65B1888903FF38CD4CCB8E4
Part of this work was supported by a Grant‐in‐Aid for Scientific Research (B) (No. 12557201) from the Japan Society for the Promotion of Science.
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ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20010601)40:11<2079::AID-ANIE2079>3.0.CO;2-Z