A 2′,4′-Bridged Nucleic Acid Containing 2-Pyridone as a Nucleobase: Efficient Recognition of a C⋅G Interruption by Triplex Formation with a Pyrimidine Motif
Significantly enhanced binding affinity to C⋅G base pairs without loss of sequence selectivity is achieved by using a nucleotide containing a 2‐pyridone and a 2′‐O,4′‐C‐methylene‐bridged nucleic acid analogue (PB, see picture). The degree of stabilization of the triplex formed enables C⋅G interrupti...
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Published in | Angewandte Chemie (International ed.) Vol. 40; no. 11; pp. 2079 - 2081 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag GmbH
01.06.2001
WILEY‐VCH Verlag GmbH |
Subjects | |
Online Access | Get full text |
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Summary: | Significantly enhanced binding affinity to C⋅G base pairs without loss of sequence selectivity is achieved by using a nucleotide containing a 2‐pyridone and a 2′‐O,4′‐C‐methylene‐bridged nucleic acid analogue (PB, see picture). The degree of stabilization of the triplex formed enables C⋅G interruptions in a homopurine⋅homopyrimidine double‐stranded DNA to be detected. |
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Bibliography: | ArticleID:ANIE2079 ark:/67375/WNG-DVP1S16D-5 istex:B73D18E763561B79A65B1888903FF38CD4CCB8E4 Part of this work was supported by a Grant‐in‐Aid for Scientific Research (B) (No. 12557201) from the Japan Society for the Promotion of Science. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/1521-3773(20010601)40:11<2079::AID-ANIE2079>3.0.CO;2-Z |