Giardia lamblia: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models

• Published in: Current protocols in microbiology (Online) Vol. 57; no. 1; p. e102
• Main Authors: Fink, Marc Y, Shapiro, Danielle, Singer, Steven M
• Format: Journal Article
• Language: English
• Published: United States 01.06.2020
• Subjects: Animals; Cell Culture Techniques - methods; Disease Models, Animal; Giardia lamblia - genetics; Giardia lamblia - growth & development; Giardia lamblia - physiology; Giardiasis - parasitology; Humans; Life Cycle Stages; Mice; Parasitology - methods; Preservation, Biological - methods; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Trophozoites - genetics; Trophozoites - growth & development; Trophozoites - physiology; Giardia lamblia; passage and maintenance; protozoan parasite; giardiasis; murine infection; animal model; cryopreservation
• ISSN: 1934-8533
• DOI: 10.1002/cpmc.102

Giardia lamblia is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. Giardia exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. Infections are typically initiated through the consumption of cyst-contaminated water or food. Giardia was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, Giardia is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain Giardia and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC. Basic Protocol 1: In vitro maintenance and growth of Giardia trophozoites Basic Protocol 2: In vitro encystation of Giardia cysts Basic Protocol 3: In vivo infections using Giardia trophozoites.