Enzyme-linked immunospot assay (ELISPOT): Quantification of Th-1 cellular immune responses against microbial antigens

• Published in: Journal of visualized experiments no. 45
• Main Authors: Chambers, Isfahan R, Cone, Tiffany R, Oswald-Richter, Kyra, Drake, Wonder P
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 23.11.2010
• Subjects: Adaptive Immunity - immunology; Antigens - immunology; Enzyme-Linked Immunospot Assay - methods; Epitopes, T-Lymphocyte - immunology; Humans; Immunology; Th1 Cells - immunology
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/2221

Adaptive immunity is an important component to clearance of intracellular pathogens. The ability to detect and quantify these responses in humans is an important diagnostic tool. The enzyme-linked immunospot assay (ELISPOT) is gaining popularity for its ability to identify cellular immune responses against microbial antigens, including immunosuppressed populations such as those with HIV infection, transplantation, and steroid use. This assay has the capacity to quantify the immune responses against specific microbial antigens, as well as distinguish if these responses are Th1 or Th2 in character. ELISPOT is not limited to the site of inflammation. It is versatile in its ability to assess for immune responses within peripheral blood, as well as sites of active involvement such as bronchoalveolar lavage, cerebral spinal fluid, and ascites. Detection of immune responses against a single or multiple antigens is possible, as well as specific epitopes within microbial proteins. This assay facilitates detection of immune responses over time, as well as distinctions in antigens recognized by host T cells. Dual color ELISPOT assays are available for detection of simultaneous expression of two cytokines. Recent applications for this technique include diagnosis of extrapulmonary tuberculosis, as well as investigation of the contribution of infectious antigens to autoimmune diseases.