Synthesis and application of glycoconjugate-functionalized magnetic nanoparticles as potent anti-adhesion agents for reducing enterotoxigenic Escherichia coli infections

• Published in: Nanoscale Vol. 7; no. 18; pp. 8326 - 8331
• Main Authors: Raval, Yash S, Stone, Roland, Fellows, Benjamin, Qi, Bin, Huang, Guohui, Mefford, O Thompson, Tzeng, Tzuen-Rong J
• Format: Journal Article
• Language: English
• Published: England 14.05.2015
• Subjects: Bacterial Adhesion - drug effects; Bacterial Adhesion - physiology; Biofilms - drug effects; Biofilms - growth & development; Coated Materials, Biocompatible - chemical synthesis; Coated Materials, Biocompatible - pharmacology; Enterotoxigenic Escherichia coli - drug effects; Enterotoxigenic Escherichia coli - physiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - prevention & control; Glycoconjugates - chemistry; Glycoconjugates - pharmacology; Humans; Magnetite Nanoparticles - administration & dosage; Magnetite Nanoparticles - chemistry; Nanoconjugates - administration & dosage; Nanoconjugates - chemistry
• ISSN: 2040-3364; 2040-3372
• DOI: 10.1039/c5nr00511f

Polyethylene oxide stabilized magnetic nanoparticles (PEO-MNPs) bio-functionalized with glycoconjugate (Neu5Ac(α2-3)Gal(β1-4)Glcβ-sp) (GM3-MNPs) are synthesized using click chemistry. Interaction of GM3-MNPs with Enterotoxigenic Escherichia coli (ETEC) strain K99 (EC K99) is investigated using different microscopic techniques. Our results suggest that GM3-MNPs can effectively act as non-antibiotic anti-adhesion agents for treating ETEC infections.