Purification and characterization of two isoforms from Candida rugosa lipase B

Two isoforms of Candida rugosalipase B (LB1 and LB2) were purified by anionic exchange chromatography. The lipases had the same N-terminal sequence, carbohydrate content and pH and thermal stability but different pIs and significant differences in their activities against different p-nitrophenol est...

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Published inBiotechnology letters Vol. 22; no. 16; pp. 1291 - 1294
Main Authors LOPEZ, Cristina, GUERRA, Nelson P, RUA, M. Luisa
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.08.2000
Springer Nature B.V
Subjects
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Candida rugosa
carbohydrates
Fundamental and applied biological sciences. Psychology
Ion chromatography
Ion exchange
Miscellaneous
Mission oriented research
Yeast
Molecular form
Yeast
Purification
Enzyme
Triacylglycerol lipase
Esterases
Carboxylic ester hydrolases
Fungi
Enzymatic activity
Substrate specificity
Hydrolases
Fungi Imperfecti
Kinetic parameter
Candida rugosa
Physicochemical properties
Thallophyta
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Summary:Two isoforms of Candida rugosalipase B (LB1 and LB2) were purified by anionic exchange chromatography. The lipases had the same N-terminal sequence, carbohydrate content and pH and thermal stability but different pIs and significant differences in their activities against different p-nitrophenol esters and triacylglycerides.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005697419227