Quantitative Analysis of Red Bone Marrow Microenvironment Cells in Patients with Chronic Myeloid Leukemia, Multiple Myeloma, and Chronic Lymphocytic Leukemia in the Dynamics of Chemotherapy

• Published in: Bulletin of experimental biology and medicine Vol. 165; no. 6; pp. 786 - 789
• Main Authors: Dolgikh, T. Yu, Kachesov, I. V., Tornuev, V. V., Vinogradova, E. V., Krinitsyna, Yu. M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2018; Springer Nature B.V
• Subjects: Adipocytes; Antineoplastic Agents - pharmacology; Biomedical and Life Sciences; Biomedicine; Biopsy; Bone marrow; Bone Marrow - pathology; Bone Marrow - physiology; Cell Biology; Cell Proliferation; Chemotherapy; Chronic lymphocytic leukemia; Chronic myeloid leukemia; Comparative analysis; Cytokines - metabolism; Female; Fibroblasts; Fibroblasts - cytology; Humans; Internal Medicine; Laboratory Medicine; Leukemia; Leukemia, Lymphocytic, Chronic, B-Cell - drug therapy; Leukemia, Lymphocytic, Chronic, B-Cell - metabolism; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - metabolism; Lymphatic leukemia; Male; Middle Aged; Multiple myeloma; Multiple Myeloma - drug therapy; Multiple Myeloma - metabolism; Myeloid leukemia; Neoplasm Recurrence, Local; Oncology; Pathology; Quantitative analysis; Stromal cells; Tumor Microenvironment; chronic myeloid leukemia; multiple myeloma; chronic lymphocytic leukemia; cells of bone marrow microenvironment
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4265-9

We performed comparative analysis of quantitative changes in the populations of bone marrow microenvironment cells in patients with chronic myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia in the debut, during response to chemotherapy, and during relapse/progression/loss of response. It was shown that in the active phase of hemoblastoses, the number of reticular cells and fibroblasts in trephine biopsy specimens was higher than in the phase of response to chemotherapy and than in the control group. In patients with relapse of multiple myeloma and loss of response in chronic myeloid leukemia, the percentage ratio of adipocytes in the bone marrow significantly (by 9-13-fold) increased. In addition, endotheliocytes appear in the active phase of all hemoblastoses in trephine biopsy specimens, while in the phase of response to chemotherapy and in the control group, these cells were absent. The revealed quantitative changes in bone marrow stromal cells can be taken into account during assessing the phase of hemoblastosis and effectiveness of chemotherapy.