Pusillimonas soli sp. nov., isolated from farm soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 60; no. Pt 10; pp. 2326 - 2330
• Main Authors: LEE, Myungjin, WOO, Sung-Geun, CHAE, Myoungsoo, TEN, Leonid N
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.10.2010
• Subjects: Alcaligenaceae - classification; Alcaligenaceae - genetics; Alcaligenaceae - isolation & purification; Alcaligenaceae - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Locomotion; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids - analysis; Phylogeny; Polyamines - analysis; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Systematics; Bacteria; New species; Taxonomy; Cultivated soil
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.020404-0

A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07(T), was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07(T) belongs to the family Alcaligenaceae, class Betaproteobacteria, and is related most closely to Pusillimonas ginsengisoli KCTC 22046(T) (98.6 % sequence similarity) and Pusillimonas noertemannii BN9(T) (96.9 %). The levels of 16S rRNA gene sequence similarity between strain MJ07(T) and members of all other recognized species of the family Alcaligenaceae were below 95.2 %. The G+C content of the genomic DNA of strain MJ07(T) was 59.4 mol%. The detection of a quinone system with ubiquinone Q-8 as the major respiratory lipoquinone, putrescine as the predominant polyamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unknown aminolipids as major polar lipids and a fatty acid profile with C₁₆:₀ (32.0 %), C₁₇:₀cyclo (24.7 %) and C₁₉:₀cyclo ω8c (11.5 %) as the major components supported the affiliation of strain MJ07(T) to the genus Pusillimonas. Strain MJ07(T) exhibited relatively low levels of DNA-DNA relatedness with respect to P. ginsengisoli KCTC 22046(T) (50 ± 8 %) and P. noertemannii KACC 13183(T) (18 ± 7 %). On the basis of its phenotypic and genotypic properties together with its phylogenetic distinctiveness, strain MJ07(T) (=KCTC 22455(T) =JCM 16386(T)) should be classified in the genus Pusillimonas as the type strain of a novel species, for which the name Pusillimonas soli sp. nov. is proposed.