Photodynamic effect of protoporphyrin diarginate (PPArg2) on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts

The worldwide rise in the antibiotic resistance of bacteria forces the development of alternative antimicrobial treatments. A potential approach is photodynamic inactivation (PDI). The aim of the present study was to determine the phototoxicity of protoporphyrin diarginate (PPArg(2)) against methici...

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Published inActa biochimica polonica Vol. 55; no. 1; pp. 85 - 90
Main Authors Grinholc, Mariusz, Kawiak, Anna, Kurlenda, Julianna, Graczyk, Alfreda, Bielawski, Krzysztof P
Format Journal Article
LanguageEnglish
Published Poland 01.01.2008
Subjects
Arginine - analogs & derivatives
Arginine - pharmacology
Dermis - cytology
Dose-Response Relationship, Drug
Drug Resistance, Bacterial
Fibroblasts - cytology
Fibroblasts - metabolism
Humans
Light
Methicillin - pharmacology
Methicillin Resistance
Models, Biological
Photochemistry - methods
Photosensitizing Agents - pharmacology
Protoporphyrins - pharmacology
Skin - cytology
Skin - metabolism
Staphylococcus aureus
Staphylococcus aureus - metabolism
Tetrazolium Salts - pharmacology
Thiazoles - pharmacology
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Summary:The worldwide rise in the antibiotic resistance of bacteria forces the development of alternative antimicrobial treatments. A potential approach is photodynamic inactivation (PDI). The aim of the present study was to determine the phototoxicity of protoporphyrin diarginate (PPArg(2)) against methicillin-resistant Staphylococcus aureus and human dermal fibroblasts. Different concentrations (0 to 20 microM) of PPArg(2) and light dose of 6 J cm(-2) were tested. Cell viability was evaluated using the methylthiazoletetrazolium (MTT) assay. Incubation with 10 microM followed by illumination yielded a 3.6 log(10)-unit reduction in the viable count for Staphylococcus aureus. At the same experimental conditions, only 22.5% of the fibroblasts were photoinactivated. Protoporphyrin diarginate at concentrations up to 20 microM demonstrated no toxicity towards S. aureus or fibroblasts when not irradiated. These results suggest that the protoporphyrin diarginate exerts a high bactericidal effect against methicillin-resistant S. aureus strain without harming eukaryotic cells.
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ISSN:0001-527X
1734-154X
DOI:10.18388/abp.2008_3204