Effect of puerarin on retinal pigment epithelial cells apoptosis induced partly by peroxynitrite via Fas/FasL pathway

• Published in: International journal of ophthalmology Vol. 3; no. 4; pp. 283 - 287
• Main Authors: Hao, Li-Na, Zhang, Yan-Qing, Shen, Yu-Hua, Wang, Zhi-Yun, Wang, Yan-Hua, Zhang, Hai-Fang, He, Shou-Zhi
• Format: Journal Article
• Language: English
• Published: China International Journal of Ophthalmology Press 2010
• Subjects: Basic Research; oxidative; retinal pigment epithelial cells; puerarin; cell signal; complement
• ISSN: 2222-3959; 2222-3959
• DOI: 10.3980/j.issn.2222-3959.2010.04.01

To evaluate the peroxynitrite (ONOO(-)) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by peroxynitrite via Fas/FasL. RPE cells from C57BL/6 mice eyes were cultured. Diabetes was induced in Sprague-Dawley (SD) rats by streptozotocin (STZ) intraperitoneal injection. Puerarin was administrated to cultured RPE cells and diabetic rats. Western blotting analysis, DNA ladder, RT-PCR, immunohistochemistry were used for determining the expression of nitrotyrosine (NT, the foot print of ONOO(-)), complement 3 (C3); apoptosis and inducible nitric oxide synthase (iNOS) mRNA as well as Fas/FasL signal transduction in RPE cells. Both RPE cells in ONOO(-) and puerarin group developed apoptosis and expressed NT, C3, iNOS mRNA and Fas/FasL. But latter delayed the all changes in a time-dependent manner compared with control and STZ group (P<0.001). iNOS, C3 and Fas/FasL were up-regulated and associated with an increase of expression of ONOO(-)in vivo and in vitro. Puerarin decreases RPE cells apoptosis partly induced by ONOO(-) for diabetic retinopathy.