Effect of the Forth and Fifth Zinc Finger Deletions of MTF-1 on the Expression of Metal Ion Metabolism Related Gene

• Published in: Doklady. Biochemistry and biophysics Vol. 500; no. 1; pp. 385 - 392
• Main Authors: Xu, Hongyan, Luo, Ying, Tu, Xinzhuo, Cui, Weitong, Dou, Ye, Wang, Qinglu
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.09.2021; Springer Nature B.V
• Subjects: Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene expression; Gene Expression Regulation; HEK293 Cells; Humans; Life Sciences; Metabolism; Metal ions; Metals - metabolism; Molecular Biology; MTF-1 protein; Mutants; Proteins; Regulatory proteins; Sequence Deletion; Transcription Factor MTF-1; Transcription Factors - genetics; Transcription Factors - metabolism; Zinc; Zinc finger proteins; Zinc Fingers; zinc finger; mutant; MTF-1; metal ion metabolism; ERR signaling
• ISSN: 1607-6729; 1608-3091; 1608-3091
• DOI: 10.1134/S1607672921050082

Metal response element binding transcription factor 1 (MTF-1) is one of the important regulatory proteins involved in the mediation of intracellular metal ion balance, which is zinc dependent. The changes of zinc finger effected its function. MTF-1 mutant 293T cell line was obtained by transferring the vector of MTF-1 4th or 5th mutant zinc finger into 293T cell line that knocked out MTF-1 gene. The results showed that the mutant of 4th zinc finger in MTF-1 protein showed a significant difference on target gene expression compared with 5th zinc finger. Further RNA-seq assay showed that 4th and 5th zinc finger of MTF-1 have a different effect on molecular biological functions, cellular components, and biological process. The mutant of 4th and 5th zinc finger in MTF-1 protein changed different signaling pathways and metal ion metabolism related to genes. The present study evaluated that 4th or 5th mutant zinc finger in MTF-1 gene were associated with the function of MTF-1 protein.