Protective Effect of Iris germanica L. Rhizome-Derived Exosome against Oxidative-Stress-Induced Cellular Senescence in Human Epidermal Keratinocytes

Plant-derived exosomes can exert therapeutic effects against various dermatological conditions. Several studies have demonstrated that plant-derived exosomes can have positive effects on the skin, preventing aging, hyperpigmentation, and hair loss. In this study, the protective effects of Iris germa...

Full description

Saved in:
Bibliographic Details
Published inApplied sciences Vol. 13; no. 21; p. 11681
Main Authors Kim, Ji-Seon, Lee, Hyun-Jeong, Yoon, Eun-Jeong, Lee, Hyunsang, Ji, Youngeun, Kim, Youngseok, Park, Si-Jun, Kim, Junoh, Bae, Seunghee
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2023
Subjects
Aging
antioxidant
Antioxidants
Apoptosis
Cancer
Dermatitis
exosome
Flavonoids
Free radicals
Iris germanica L. rhizome
keratinocytes
Lipids
Metabolites
Molecular weight
Nanoparticles
Oxidative stress
Physiology
Senescence
Skin diseases
Tumor necrosis factor-TNF
St Louis Missouri
United States > US
Switzerland
Online AccessGet full text

Cover

More Information
Summary:Plant-derived exosomes can exert therapeutic effects against various dermatological conditions. Several studies have demonstrated that plant-derived exosomes can have positive effects on the skin, preventing aging, hyperpigmentation, and hair loss. In this study, the protective effects of Iris germanica L. rhizome-derived exosomes (Iris-exosomes) on oxidative-stress-induced cellular dysfunction were investigated in human epidermal keratinocytes (nHEKs). Iris-exosomes with a diameter range of 100–300 nm were detected. In the cytotoxicity assay, Iris-exosomes with up to 107 particles per milliliter were found to possess no cytotoxicity, and we recovered H2O2-induced cell viability loss. In nHEKs, H2O2-induced ROS levels were significantly reduced using Iris-exosomes and additionally associated with increases in antioxidant enzyme transcription. The H2O2-induced SA-β-gal-positive nHEKs were decreased using Iris-exosomes; these effects correlate with the changed levels of cell cycle arrest marker p21. Furthermore, the H2O2-induced loss of in vitro wound-healing properties and early detection of keratin 1 and 10—keratinization markers—were restored to control levels using Iris-exosomes. Altogether, these results indicate the possibility that Iris-exosomes exert antioxidant and anti-senescence effects in order to protect against oxidative-stress-induced cellular dysfunction in nHEKs.
ISSN:2076-3417
2076-3417
DOI:10.3390/app132111681