Fingerprinting Trichoderma reesei hydrolases in a commercial cellulase preparation

• Published in: Applied biochemistry and biotechnology Vol. 91; no. 1-9; pp. 99 - 107
• Main Authors: Vinzant, T.B, Adney, W.S, Decker, S.R, Baker, J.O, Kinter, M.T, Sherman, N.E, Fox, J.W, Himmel, M.E
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Heidelberg Springer 2001; Springer Nature B.V
• Subjects: Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; biomass; Biotechnology; Carbohydrate Metabolism; Cellulase - chemistry; Cellulase - genetics; Cellulase - isolation & purification; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; endo-1,4-beta-glucanase; Enzymes; Ethanol - metabolism; Fundamental and applied biological sciences. Psychology; gels; genes; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - genetics; Glycoside Hydrolases - isolation & purification; Hypocrea jecorina; lignocellulose; Mass Spectrometry; Miscellaneous; Mission oriented research; Peptide Mapping; Proteome; sugars; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma reesei; two-dimensional gel electrophoresis; Enzyme; Liquid chromatography; Lignocellulosics; Biomass; Trichoderma reesei; Fungi; Analysis method; Cellulase; Glycosidases; Biotransformation; Mass spectrometry MS/MS; Hydrolases; Fungi Imperfecti; Two dimensional electrophoresis; O-Glycosidases; Application; Aminoacid sequence; Thallophyta
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:91-93:1-9:99

Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 x 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.