Visualizing the molecular mode of motion from a correlative analysis of localization microscopy datasets
The determination of the mode and rapidity of motion of individual molecules within a biological sample is becoming a more and more common analysis in biophysical investigations. Single molecule tracking (SMT) techniques allow reconstructing the trajectories of individual molecules within a movie, p...
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| Published in | Optics letters Vol. 41; no. 19; p. 4503 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
United States
01.10.2016
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| Online Access | Get more information |
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| Summary: | The determination of the mode and rapidity of motion of individual molecules within a biological sample is becoming a more and more common analysis in biophysical investigations. Single molecule tracking (SMT) techniques allow reconstructing the trajectories of individual molecules within a movie, provided that the position from one frame to the other can be correctly linked. The outcomes, however, appear to depend on the specific method used, and most techniques display a limitation to capture fast modes of motion in a crowded environment. We demonstrate here that the limitations encountered by conventional SMT can be significantly overcome by employing alternative approaches based on image spatial-temporal correlations, enabling to visually extract quantitative insights on the ensemble mode of motion of fluorescently labeled biomolecules that would otherwise be inaccessible. |
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| ISSN: | 1539-4794 |
| DOI: | 10.1364/OL.41.004503 |