Rapid identification of monocytes in a mixed mononuclear cell preparation

A method for identifying monocytes by the "non-specific esterase" stain is described. This method is particularly applicable to mononuclear cell suspensions obtained by Ficoll--Hypaque density gradient separations and allows rapid as well as accurate determinations.

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Bibliographic Details
Published inJournal of immunological methods Vol. 14; no. 3-4; p. 267
Main Authors Tucker, S B, Pierre, R V, Jordon, R E
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1977
Subjects
Cell Separation - methods
Centrifugation, Density Gradient
Monocytes
Staining and Labeling
Time Factors
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Summary:A method for identifying monocytes by the "non-specific esterase" stain is described. This method is particularly applicable to mononuclear cell suspensions obtained by Ficoll--Hypaque density gradient separations and allows rapid as well as accurate determinations.
ISSN:0022-1759
DOI:10.1016/0022-1759(77)90137-5