On-line biomass monitoring in bench-scale stirred bioreactors using parts of a liquid chromatography system

A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l^sup -1^. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation...

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Bibliographic Details
Published inBiotechnology letters Vol. 24; no. 23; pp. 1999 - 2003
Main Authors FORCATO, Diego O, PECORA, Rolando P, KIVATINITZ, Silvia C
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.12.2002
Springer Nature B.V
Subjects
Bacteria
Biological and medical sciences
Bioreactors
Biotechnology
Fermentation
Fundamental and applied biological sciences. Psychology
Liquid chromatography
Methods. Procedures. Technologies
Various methods and equipments
benchtop bioreactors
Bioreactor
biomass
On line
real-time
Liquid chromatography
Fermentation
Monitoring
on-line monitoring
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Summary:A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l^sup -1^. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l^sup -1^), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1021110730741