Line-scanning detection instrument for photonic crystal enhanced fluorescence

A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating...

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Published inOptics letters Vol. 37; no. 13; p. 2565
Main Authors Chaudhery, Vikram, Lu, Meng, Huang, Cheng-Sheng, Polans, James, Tan, Ruimin, Zangar, Richard C, Cunningham, Brian T
Format Journal Article
LanguageEnglish
Published United States 01.07.2012
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Abstract A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.
AbstractList A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.
Author Lu, Meng
Tan, Ruimin
Polans, James
Chaudhery, Vikram
Zangar, Richard C
Huang, Cheng-Sheng
Cunningham, Brian T
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/22743456$$D View this record in MEDLINE/PubMed
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Snippet A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of...
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StartPage 2565
SubjectTerms Biomarkers, Tumor - analysis
Nanostructures
Photons
Protein Array Analysis
Spectrometry, Fluorescence - instrumentation
Surface Properties
Title Line-scanning detection instrument for photonic crystal enhanced fluorescence
URI https://www.ncbi.nlm.nih.gov/pubmed/22743456
Volume 37
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