Line-scanning detection instrument for photonic crystal enhanced fluorescence

• Published in: Optics letters Vol. 37; no. 13; p. 2565
• Main Authors: Chaudhery, Vikram, Lu, Meng, Huang, Cheng-Sheng, Polans, James, Tan, Ruimin, Zangar, Richard C, Cunningham, Brian T
• Format: Journal Article
• Language: English
• Published: United States 01.07.2012
• Subjects: Biomarkers, Tumor - analysis; Nanostructures; Photons; Protein Array Analysis; Spectrometry, Fluorescence - instrumentation; Surface Properties
• ISSN: 1539-4794
• DOI: 10.1364/OL.37.002565

A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.