Genomic Characterization of a Uropathogenic Escherichia coli ST405 Isolate Harboring blaCTX-M-15-Encoding IncFIA-FIB Plasmid, blaCTX-M-24-Encoding IncI1 Plasmid, and Phage-Like Plasmid
Escherichia coli sequence type 405 is an emerging antibiotic-resistant clonal group associated with the global dissemination of extended-spectrum β-lactamase-producing E. coli . In this study, we report the genome assembly and characterization of a uropathogenic E. coli ST405 strain, SZESBLEC201, ba...
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Published in | Frontiers in microbiology Vol. 13 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
11.04.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Escherichia coli
sequence type 405 is an emerging antibiotic-resistant clonal group associated with the global dissemination of extended-spectrum β-lactamase-producing
E. coli
. In this study, we report the genome assembly and characterization of a uropathogenic
E. coli
ST405 strain, SZESBLEC201, based on long and short reads obtained from the Nanopore and Illumina sequencing platforms, respectively. Whole-genome sequencing revealed that SZESBLEC201 harbors a 5,020,403 bp chromosome and three plasmids, namely, pSZESBLEC201-1, pSZESBLEC201-2, and pSZESBLEC201-3. pSZESBLEC201-1 (111,621 bp) belongs to the IncFIA-FIB type and harbors
bla
CTX-M-15
. However, this plasmid does not harbor conjugative transfer-associated genes, rendering pSZESBLEC201-1 unable to be conjugatively transferred. pSZESBLEC201-2 (95,138 bp) is a phage-like plasmid that shows a strong genome synteny with
Escherichia
phage P1 but with the absence of mobile genetic elements and some regulatory genes. pSZESBLEC201-3 (92,865 bp) belongs to the IncI1 type and carries
bla
CTX-M-24
. In contrast to pSZESBLEC201-1, pSZESBLEC201-3 retains its full active conjugation machinery and can be transferred
via
conjugation. The genetic features of the genome show that the SZESBLEC201 has a unique virulence pattern compared with genetically similar strains found in the same country (China). The plasmid backbones exhibit a high degree of similarity to those of geographically distant isolates, highlighting the global spread of
bla
CTX-M
genes and the genome plasticity of this clonal group. The coexistence of two
bla
CTX-M
variants in the same strain increases the risk of the emergence of new
bla
CTX-M
variants. Further studies on phage-like plasmids are necessary to provide insights into their biological activities and clinical significance. |
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Bibliography: | Edited by: Axel Cloeckaert, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), France Reviewed by: Joost Hordijk, National Institute for Public Health and the Environment, Netherlands; Rafael Vignoli, Universidad de la República, Uruguay; Roger Stephan, University of Zurich, Switzerland These authors have contributed equally to this work and share first authorship This article was submitted to Infectious Agents and Disease, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.845045 |