Murine B1 B Cells Require IL-5 for Optimal T Cell-Dependent Activation

• Published in: The Journal of immunology (1950) Vol. 166; no. 3; pp. 1531 - 1539
• Main Authors: Erickson, Loren D, Foy, Teresa M, Waldschmidt, Thomas J
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.02.2001
• Subjects: Animals; Antibodies, Blocking - pharmacology; Ascitic Fluid - cytology; Ascitic Fluid - immunology; Ascitic Fluid - metabolism; B-Lymphocyte Subsets - cytology; B-Lymphocyte Subsets - immunology; B-Lymphocyte Subsets - metabolism; CD40 antigen; CD40 Antigens - biosynthesis; CD40 Ligand - genetics; CD40 Ligand - immunology; CD40 Ligand - pharmacology; CD40L protein; Cell Differentiation - genetics; Cell Differentiation - immunology; Cells, Cultured; Clone Cells; Coculture Techniques; Female; Immune Sera - pharmacology; Immunoglobulin Class Switching - immunology; Immunoglobulins - biosynthesis; Interleukin-4 - pharmacology; Interleukin-5 - immunology; Interleukin-5 - pharmacology; Interleukin-5 - physiology; Lymphocyte Activation - immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, Knockout; Recombinant Proteins - pharmacology; Th2 Cells - immunology; Th2 Cells - metabolism
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.166.3.1531

T helper cell-driven activation of murine B cells has been shown to depend upon CD40-CD40 ligand (CD40L) interactions and a defined set of cytokines. These observations are primarily based on the use of conventional B cells obtained from the spleen. Therefore, it is presently unclear whether all mature B cell subsets found in the mouse have an equal dependence upon CD40-CD40L interactions and use the same T cell-derived cytokines. The present study tested the response of splenic follicular and marginal zone as well as peritoneal B2 and B1 B cells to Th cell stimulation. Splenic and peritoneal B cell subsets were sort purified based on CD23 expression, and cultured with rCD40L and cytokines or Th2 cells. The results demonstrate that follicular, marginal zone, and peritoneal B2 B cells require CD40-CD40L interactions and preferentially use IL-4 for optimal proliferation, differentiation, and isotype switching. In contrast, peritoneal B1 B cells use IL-5 in conjunction with CD40-CD40L interactions for maximal Th cell-dependent responses. Furthermore, B1 B cells are capable of proliferating, differentiating, and isotype switching in the absence of CD40-CD40L interactions. B1 B cells are able to respond to Th2 clones in the presence of anti-CD40L mAb as well as to Th2 clones derived from CD40L(-/-) mice. The CD40-CD40L-independent response of B1 B cells is attributable to the presence of both IL-4 and IL-5, and may explain the residual Ab response to T cell-dependent Ags in CD40L- or CD40-deficient mice, and in X-linked hyper-IgM (X-HIM) patients.