Laparoscopy/laparotomy oocyte recovery and juvenile breeding

• Published in: Animal reproduction science Vol. 42; no. 1; pp. 227 - 238
• Main Author: Tervit, H.R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.04.1996
• Subjects: ABDOMEN; AMELIORATION DES ANIMAUX; ANIMAL BREEDING; ANIMAL DOMESTIQUE; ANIMAL EMBRYOS; ANIMALES DOMESTICOS; DOMESTIC ANIMALS; EMBRIONES ANIMALES; EMBRYON ANIMAL; ENDOSCOPIA; ENDOSCOPIE; ENDOSCOPY; FORMAS JUVENILES; In vitro embryos; JUVENILE; JUVENILES; Laparoscopy; LAPAROTOMIA; LAPAROTOMIE; LAPAROTOMY; MEJORAMIENTO ANIMAL; Oocytes; OVA; OVULE; OVULO; Laparoscopy; In vitro embryos; Laparotomy; Juveniles; Oocytes
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/0378-4320(96)01533-3

This paper reviews the status and use of laparoscopic and laparotomy oocyte recovery technology with particular emphasis on domestic animals. The technology enables the recovery of oocytes from species and ages where it is not possible, or easy, to conduct recoveries through the vagina. It potentially has a powerful role in increasing genetic progress through in vitro production (IVP) of embryos from juveniles. Calves can be induced to produce large numbers of follicies, but the recovered oocytes show variable development in vitro and in vivo compared with cow oocytes. Lambs can produce up to 180 mature oocytes which could result in up to 25 offspring from a single collection. Technology developed in mature cattle gives similar numbers of oocytes to transvaginal recoveries (TVR) but, because the technique is more invasive than TVR, is less frequently used. Laparoscopic oocyte retrieval in sheep results in one to three good quality blastocysts and 1.5 lambs born per ewe aspirated. This level of performance is useful for producing offspring from infertile ewes but needs improvement before it replaces multiple ovulation embryo transfer (MOET). Application of the technology to other species is primarily dependent on developing efficient IVP procedures.