Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida : influence of calcium chloride, hemicellulase and theophylline

CaCl^sub 2^ (50 mM) and hemicellulase (0.5 U mg^sup -1^) increased the intracellular accumulation (60-250%), release (60-200%) and production (45-200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida. Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffec...

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Published inBiotechnology letters Vol. 22; no. 20; pp. 1653 - 1656
Main Authors PITTA-ALVAREZ, S. L, SPOLLANSKY, T. C, GIULIETTI, A. M
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.10.2000
Springer Nature B.V
Subjects
Biological and medical sciences
Biotechnology
Brugmansia candida
Calcium
Calcium chloride
Cell culture
Fundamental and applied biological sciences. Psychology
hemicellulase
hyoscyamine
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
scopolamine
theophylline
Yeast
Hyoscyamine
Cell culture
Hairy root
Hyoscine
Vegetals
Enzyme
Elicitor
Calcium Chlorides
Hemicellulase
Dicotyledones
Angiospermae
Production
Hydrolases
Spermatophyta
Solanaceae
Intracellular
Release
Theophylline
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Summary:CaCl^sub 2^ (50 mM) and hemicellulase (0.5 U mg^sup -1^) increased the intracellular accumulation (60-250%), release (60-200%) and production (45-200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida. Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffective in increasing hyoscyamine and scopolamine production.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005638029034