An optical coherence microscope for 3-dimensional imaging in developmental biology

An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 1...

Full description

Saved in:
Bibliographic Details
Published inOptics express Vol. 6; no. 7; pp. 136 - 146
Main Authors Hoeling, B, Fernandez, A, Haskell, R, Huang, E, Myers, W, Petersen, D, Ungersma, S, Wang, R, Williams, M, Fraser, S
Format Journal Article
LanguageEnglish
Published United States 27.03.2000
Online AccessGet full text

Cover

More Information
Summary:An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.6.000136