Spectrophometric analysis of volatile compounds in microorganisms

A simple modification of a spectrophometric method was proposed for the rapid detection of microorganisms based on their ability either to excrete or to absorb volatile compounds. The method provides the possibility of contactless control for bacterial growth at a concentration above 10⁷ cells/ml. I...

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Published inApplied biochemistry and microbiology Vol. 46; no. 3; pp. 303 - 306
Main Authors Voloshin, A. G, Filippovich, S. Yu, Bachurina, G. P, Besaeva, S. G, Ignatov, S. G
Format Journal Article
LanguageEnglish
Published Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.05.2010
SP MAIK Nauka/Interperiodica
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Chemical compounds
Life Sciences
Medical Microbiology
Microbiology
Microorganisms
Mutation
Neurospora crassa
Spectrum analysis
Neurospora Crassa
Logarithmic Growth Phase
Nitrite Reductase
Apply Biochemistry
Volatile Compound
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Summary:A simple modification of a spectrophometric method was proposed for the rapid detection of microorganisms based on their ability either to excrete or to absorb volatile compounds. The method provides the possibility of contactless control for bacterial growth at a concentration above 10⁷ cells/ml. In addition, the method allows discriminating mutants of the fungus Neurospora crassa defective in the nitrogen metabolism from the wild type strains. It is likely that nitrite reductase and nitrate reductase enzymes regulated by the nit-2 and nit-6 genes are involved in formation of the water soluble volatile compounds of this organism.
Bibliography:http://dx.doi.org/10.1134/S0003683810030099
ObjectType-Article-2
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ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683810030099