Immobilization of endo-polygalacturonase from Aspergillus ustus on silica gel
Endo-polygalacturonase from Aspergillus ustus when immobilized on to modified silica gel retained 28% of its original activity. The immobilized enzyme could be re-used through 10 cycles of reaction with almost 90% retention of its original activity. It had increased thermostability over its soluble...
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Published in | Biotechnology letters Vol. 22; no. 19; pp. 1557 - 1559 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer
01.10.2000
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Endo-polygalacturonase from Aspergillus ustus when immobilized on to modified silica gel retained 28% of its original activity. The immobilized enzyme could be re-used through 10 cycles of reaction with almost 90% retention of its original activity. It had increased thermostability over its soluble form: the half-life of the soluble enzyme at 40 °C was less than 10 h whereas the immobilized enzyme retained 82% of its activity after 10 h at 40 °C. Similarly, at 50 °C the half-life of the soluble enzyme was 30 min whereas that of the immobilized enzyme was 5 h.[PUBLICATION ABSTRACT] |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1023/A:1005632917259 |