Immobilization of endo-polygalacturonase from Aspergillus ustus on silica gel

Endo-polygalacturonase from Aspergillus ustus when immobilized on to modified silica gel retained 28% of its original activity. The immobilized enzyme could be re-used through 10 cycles of reaction with almost 90% retention of its original activity. It had increased thermostability over its soluble...

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Bibliographic Details
Published inBiotechnology letters Vol. 22; no. 19; pp. 1557 - 1559
Main Authors NARSIMHA RAO, M, KEMBHAVI, A. A, PANT, A
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.10.2000
Springer Nature B.V
Subjects
Aspergillus ustus
Biological and medical sciences
Biotechnology
Enzymes
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Silica
Enzyme
Aspergillus ustus
Immobilization
Soluble form
Polygalacturonase
Silica
Thermal stability
Fungi
Enzymatic activity
Glycosidases
Hydrolases
Fungi Imperfecti
O-Glycosidases
Immobilized enzyme
Thallophyta
Half life
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Summary:Endo-polygalacturonase from Aspergillus ustus when immobilized on to modified silica gel retained 28% of its original activity. The immobilized enzyme could be re-used through 10 cycles of reaction with almost 90% retention of its original activity. It had increased thermostability over its soluble form: the half-life of the soluble enzyme at 40 °C was less than 10 h whereas the immobilized enzyme retained 82% of its activity after 10 h at 40 °C. Similarly, at 50 °C the half-life of the soluble enzyme was 30 min whereas that of the immobilized enzyme was 5 h.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005632917259