SetDB1 and Su(var)3-9 play non-overlapping roles in somatic cell chromosomes of Drosophila melanogaster

We explored functional roles of two H3K9-specific histone methyltransferases of , SetDB1 (also known as Eggless) and Su(var)3-9. Using the DamID approach, we generated the binding profile for SetDB1 in salivary gland chromosomes, and matched it to the profile of Su(var)3-9. Unlike Su(var)3-9, SetDB1...

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Published inJournal of cell science Vol. 134; no. 2
Main Authors Kalashnikova, Darya A, Maksimov, Daniil A, Romanov, Stanislav E, Laktionov, Petr P, Koryakov, Dmitry E
Format Journal Article
LanguageEnglish
Published England 22.01.2021
Subjects
Animals
Chromatin - genetics
Chromosomes
Drosophila
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Histone-Lysine N-Methyltransferase
Microtubule-Associated Proteins
Nuclear Proteins
Repressor Proteins
H3K27 methylation
Su(var)3-9
Drosophila
SetDB1
TAD
H3K9 methylation
CP190
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Summary:We explored functional roles of two H3K9-specific histone methyltransferases of , SetDB1 (also known as Eggless) and Su(var)3-9. Using the DamID approach, we generated the binding profile for SetDB1 in salivary gland chromosomes, and matched it to the profile of Su(var)3-9. Unlike Su(var)3-9, SetDB1 turned out to be an euchromatic protein that is absent from repeated DNA compartments, and is largely restricted to transcription start sites (TSSs) and 5' untranslated regions (5'UTRs) of ubiquitously expressed genes. Significant SetDB1 association is also observed at binding sites for the insulator protein CP190. SetDB1 and H3K9 di- and tri-methylated (me2 and me3)-enriched sites tend to display poor overlap. At the same time, SetDB1 has a clear connection with the distribution of H3K27me3 mark. SetDB1 binds outside the domains possessing this modification, and about half of the borders of H3K27me3 domains are decorated by SetDB1 together with actively transcribed genes. On the basis of poor correlation between the distribution of SetDB1 and H3K9 methylation marks, we speculate that, in somatic cells, SetDB1 may contribute to the methylation of a broader set of chromosomal proteins than just H3K9. In addition, SetDB1 can be expected to play a role in the establishment of chromatin functional domains.
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ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.253096