Determination of Foetal Calf Serum in Vaccine Preparations by Immunoradiometric Assay and Enzyme-linked Immunosorbent Assay
A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum proteins in vaccine preparations has been developed. In this two-site sandwich assay the same preparation of polyclonal anti-FCS immunoglobulin (IgG) was used for coating the solid phase (as a capture antibody)...
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Published in | Biologicals Vol. 21; no. 2; pp. 163 - 168 |
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Main Authors | , , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Kent
Elsevier Ltd
01.06.1993
Elsevier Science |
Subjects | |
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Abstract | A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum proteins in vaccine preparations has been developed. In this two-site sandwich assay the same preparation of polyclonal anti-FCS immunoglobulin (IgG) was used for coating the solid phase (as a capture antibody) and in a labelled form (I-125 labelled) for detection. The developed assay allows precise and accurate quantification of FCS proteins (down to 10 ng/ml) in vaccine preparations and has been used for screening of FCS residues (a) during production of measles vaccine, and (b) in various different commercial vaccine preparations. An enzyme-linked immunosorbent assay (ELISA) was also developed based on the same assay two-site sandwich principle, where anti-FCS was directly labelled with horse-radish peroxidase. ELISA was comparable in sensitivity and accuracy with IRMA. |
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AbstractList | A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum proteins in vaccine preparations has been developed. In this two-site sandwich assay the same preparation of polyclonal anti-FCS immunoglobulin (IgG) was used for coating the solid phase (as a capture antibody) and in a labelled form (I-125 labelled) for detection. The developed assay allows precise and accurate quantification of FCS proteins (down to 10 ng/ml) in vaccine preparations and has been used for screening of FCS residues (a) during production of measles vaccine, and (b) in various different commercial vaccine preparations. An enzyme-linked immunosorbent assay (ELISA) was also developed based on the same assay two-site sandwich principle, where anti-FCS was directly labelled with horse-radish peroxidase. ELISA was comparable in sensitivity and accuracy with IRMA. |
Author | Tresćec, Anda Tomasić, Jelka Valinger, Zdenka Smerdel, Stanislav |
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Keywords | Bovine Radiolabelling Antibody Vaccine Method Serum protein Immunological method Vertebrata Mammalia Fetus Artiodactyla Detection ELISA assay Ungulata Quantitative analysis |
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Snippet | A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum proteins in vaccine preparations has been developed. In this two-site... |
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SubjectTerms | Applied microbiology Biological and medical sciences Enzyme-Linked Immunosorbent Assay - methods Fetal Blood Fundamental and applied biological sciences. Psychology Immunoradiometric Assay - methods Measles Vaccine - chemistry Microbiology Rubella Vaccine - chemistry Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
Title | Determination of Foetal Calf Serum in Vaccine Preparations by Immunoradiometric Assay and Enzyme-linked Immunosorbent Assay |
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