Determination of Foetal Calf Serum in Vaccine Preparations by Immunoradiometric Assay and Enzyme-linked Immunosorbent Assay

• Published in: Biologicals Vol. 21; no. 2; pp. 163 - 168
• Main Authors: Tresćec, Anda, Valinger, Zdenka, Smerdel, Stanislav, Tomasić, Jelka
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kent Elsevier Ltd 01.06.1993; Elsevier Science
• Subjects: Applied microbiology; Biological and medical sciences; Enzyme-Linked Immunosorbent Assay - methods; Fetal Blood; Fundamental and applied biological sciences. Psychology; Immunoradiometric Assay - methods; Measles Vaccine - chemistry; Microbiology; Rubella Vaccine - chemistry; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Bovine; Radiolabelling; Antibody; Vaccine; Method; Serum protein; Immunological method; Vertebrata; Mammalia; Fetus; Artiodactyla; Detection; ELISA assay; Ungulata; Quantitative analysis
• ISSN: 1045-1056; 1095-8320
• DOI: 10.1006/biol.1993.1069

A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum proteins in vaccine preparations has been developed. In this two-site sandwich assay the same preparation of polyclonal anti-FCS immunoglobulin (IgG) was used for coating the solid phase (as a capture antibody) and in a labelled form (I-125 labelled) for detection. The developed assay allows precise and accurate quantification of FCS proteins (down to 10 ng/ml) in vaccine preparations and has been used for screening of FCS residues (a) during production of measles vaccine, and (b) in various different commercial vaccine preparations. An enzyme-linked immunosorbent assay (ELISA) was also developed based on the same assay two-site sandwich principle, where anti-FCS was directly labelled with horse-radish peroxidase. ELISA was comparable in sensitivity and accuracy with IRMA.