MCP-1 is highly expressed in peritoneum following midline laparotomy with peritoneal abrasion in a murine model

• Published in: Surgical endoscopy Vol. 16; no. 7; pp. 1079 - 1082
• Main Authors: BRODSKY, J. A, BRODY, F. J, ENDLICH, B, ARMSTRONG, D. A, PONSKY, J. L, HAMILTON, I. A
• Format: Conference Proceeding Journal Article
• Language: English
• Published: New York, NY Springer 01.07.2002; Springer Nature B.V
• Subjects: Abdomen; Animals; Biological and medical sciences; Cell Movement - physiology; Chemokine CCL2 - biosynthesis; Chemokine CCL2 - metabolism; Chemokine CCL2 - physiology; Chemokines; Cytokines; Female; Kinases; Laparotomy; Laparotomy - methods; Leukocytes; Leukocytes - pathology; Medical sciences; Mice; Mice, Inbred C57BL; Peritoneal Cavity - pathology; Peritoneal Cavity - surgery; Peritoneum - metabolism; Peritoneum - pathology; Peritoneum - surgery; Proteins; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Up-Regulation - physiology; Wound healing; Wound Healing - physiology; Abrasion; Rat; Secretion; Rodentia; Experimental study; Abdomen; Vertebrata; Mammalia; Surgery; Animal; Abdominal wall; Biological effect; Laparotomy; Monocyte chemoattractant protein 1; Peritoneum
• ISSN: 0930-2794; 1432-2218
• DOI: 10.1007/s00464-001-8335-z

Monocyte chemoattractant protein-1 (MCP-1), a CC chemokine, is a potent attractant of monocytes both in vitro and in vivo. However, its role in the repair of peritoneal injury is not well established. This study characterizes MCP-1 expression in surgical wounds following peritoneal abrasion in a murine model. Twenty-five C57 BL6 female mice underwent a 2-cm midline laparotomy with mechanical abrasion of the right peritoneal wall. The mice were sacrificed at various times ranging from 0 to 7 days. Hemotoxylin and eosin stained sections and tissue extracts were made using peritoneal samples from abraded and unabraded areas in each mouse. An enzyme-linked immunosorbent assay was performed on the specimens to quantitate MCP-1 expression. Values were compared using a t-test. At baseline, there was minimal expression of MCP-1 (<5 pg/mg protein). Following surgery, MCP-1 levels at abraded sites were significantly higher than those at both baseline and unabraded sites at all times up to a week following surgery. Histologic evaluation revealed peritoneal thickening and leukocytic infiltration of only abraded surfaces. MCP-1 is highly expressed in peritoneum following laparotomy with peritoneal abrasion. Elevations in MCP-1 levels are identified within 6 h of surgery and persist for up to 1 week. The histologic differences between abraded and unabraded areas may be attributable to differences in MCP-1 expression. Further studies using recombinant MCP-1 and anti-MCP-1 antibody may elucidate this relationship.