Isolation of a hyperxylanolytic Cellulomonas flavigena mutant growing on continuous culture on sugar cane bagasse

Mutant M9-82 from Cellulomonas flavigena PN-120 was obtained by combined treatment of NTG and EMS. Xylanase activity of this mutant strain growing on batch culture, was 33.5 IU/ml, 2.7 times higher than its parent strain. When mutant M9-82 was grown on continuous culture at a dilution rate of 0.05 h...

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Bibliographic Details
Published inBiotechnology letters Vol. 20; no. 5; pp. 443 - 446
Main Authors MAYORGA-REYES, L, PONCE-NOYOLA, T
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.05.1998
Springer Nature B.V
Subjects
Bacteriology
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic technics
Methods. Procedures. Technologies
Microbiology
Mutant screening
Prokaryotes
Culture medium
Cellulomonas flavigena
Enzyme
Xylanolytic enzyme
Continuous process
Sugar cane(by product)
Enzymatic activity
Mutagenesis
Cellulomonadaceae
Microorganism culture
Bacteria
Isolation
Actinomycetes
Mutation
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Summary:Mutant M9-82 from Cellulomonas flavigena PN-120 was obtained by combined treatment of NTG and EMS. Xylanase activity of this mutant strain growing on batch culture, was 33.5 IU/ml, 2.7 times higher than its parent strain. When mutant M9-82 was grown on continuous culture at a dilution rate of 0.05 h^sup -1^, xylanolytic activity increased to 45 IU/ml.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005423509856