High yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris

• Published in: Journal of interferon & cytokine research Vol. 16; no. 2; p. 119
• Main Authors: Van Heeke, G, Ott, T L, Strauss, A, Ammaturo, D, Bazer, F W
• Format: Journal Article
• Language: English
• Published: United States 01.02.1996
• Subjects: Animals; Base Sequence; Cell Line, Transformed; Chromosome Mapping; Culture Media; Fermentation; Interferon Type I - genetics; Interferon Type I - isolation & purification; Interferon Type I - physiology; Molecular Sequence Data; Pichia; Plasmids - genetics; Pregnancy Proteins - genetics; Pregnancy Proteins - isolation & purification; Pregnancy Proteins - physiology; Recombinant Proteins - biosynthesis; Recombinant Proteins - isolation & purification; Secretory Rate; Sheep
• ISSN: 1079-9907
• DOI: 10.1089/jir.1996.16.119

The early conceptus (embryo and associated membranes) of domestic ruminats signals its presence to the maternal uterus through production of interferon-tau (IFN-tau). Production of IFN-tau ensures continued production of progesterone, the hormone of pregnancy, by the ovarian corpus luteum. This paper reports the high-level expression and efficient secretion of biologically active recombinant ovine IFN-tau (rOvIFN-tau) by Pichia pastoris. The developed method produces more than 80% pure recombinant ovine IFN-tau, obviating the need for further purification for many purposes. Initial fermentation studies produced IFN-tau at 280 mg/liter and demonstrate the potential of this system for large-scale production of IFN-tau.