FBXW7 Mutations Promote Cell Proliferation, Migration, and Invasion in Cervical Cancer

• Published in: Genetic testing and molecular biomarkers Vol. 23; no. 6; p. 409
• Main Authors: Liu, Faying, Zou, Yang, Wang, Feng, Yang, Bicheng, Zhang, Ziyu, Luo, Yong, Liang, Meirong, Zhou, Jiangyan, Huang, Ouping
• Format: Journal Article
• Language: English
• Published: United States 01.06.2019
• Subjects: Adult; Aged; Asian Continental Ancestry Group - genetics; Carcinoma, Squamous Cell - genetics; Cell Cycle Proteins; Cell Movement - genetics; Cell Proliferation - genetics; China; F-Box Proteins; F-Box-WD Repeat-Containing Protein 7 - genetics; F-Box-WD Repeat-Containing Protein 7 - physiology; Female; Gene Expression Regulation, Neoplastic - genetics; Humans; Middle Aged; Mutation - genetics; Neoplasm Invasiveness - genetics; Uterine Cervical Neoplasms - genetics; China; migration; mutations; cell proliferation; invasion; cervical cancer
• ISSN: 1945-0257
• DOI: 10.1089/gtmb.2018.0278

Cervical cancer is the most common gynecological cancer. Recent studies have revealed that the F-box and WD repeat domain containing 7 ( ) gene, which encodes a subunit of Skp1-Cul1-F-box protein (SCF) ubiquitin ligase, is frequently mutated in cervical squamous cell carcinomas. In this study, we investigated whether Chinese cervical cancer cells also harbor these mutations. Using PCR and sequencing assays, a total of 190 specimens from Han Chinese patients with cervical cancer were analyzed for mutations. Two mutations (p.R479P and p.L443H), were identified from a study of 145 (1.4%) cervical squamous cell carcinomas. The p.L443H somatic mutation has not been previously reported. Functional assays showed that both of these mutations could promote cell proliferation, migration, and invasion. A low frequency (1.4%) of cervical squamous cell carcinomas were identified with mutations. We did, however, identify a novel mutation. Our results also demonstrated that the identified mutations could promote cell proliferation, migration, and invasion in cervical cancer cells.