Simultaneous determination of four vasoactive phytochemicals in different pharmaceutical preparations by a simple HPLC-DAD method

• Published in: Analytical methods Vol. 8; no. 8; pp. 1858 - 1866
• Main Authors: Ibrahim, Fawzia, Sharaf El-Din, Mohie Khaled, El-Shaheny, Rania Nabih, El-Deen, Asmaa Kamal, Shimizu, Kuniyoshi
• Format: Journal Article
• Language: English
• Published: 01.01.2016
• Subjects: Acetonitrile; Arrays; Buffers; Diodes; Mathematical analysis; Particle size; Pharmaceuticals; Tablets
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c5ay03003j

A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations. Separation of the four compounds was achieved in about 8 min on a phenomenex-C 18 column (250 mm × 4.6 mm, 5 μm particle size) using a mobile phase consists of 50 mM phosphate buffer (pH 4.0) : acetonitrile (75 : 25, v/v) eluted at 1.0 mL min −1 with diode array detection at 275 nm. Linear correlation was obeyed over the concentration ranges of 2.0-60.0, 1.0-20.0, 2.0-50.0, and 5.0-100.0 μg mL −1 for ASC, RUT, HSP, and DSM, respectively. The developed method is efficient, reproducible and appropriate as a versatile method for quality control of various combinations of these drugs in different pharmaceutical preparations with high values of %recoveries which agreed well with the labeled claim. The method is also a stability-indicating one for DSM in its pure state and single-component tablets. A comparison of the results obtained by the developed method with those of the comparison methods confirmed no significant differences between them with respect to accuracy and precision. A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations.