Hepatocyte cryopreservation:Is it time to change the strategy?

Liver cell transplantation presents clinical benefit in patients with inborn errors of metabolism as an alternative,or at least as a bridge,to orthotopic liver transplantation.The success of such a therapeutic approach remains limited by the quality of the transplanted cells.Cryopreservation remains...

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Published inWorld journal of gastroenterology : WJG Vol. 16; no. 1; pp. 1 - 14
Main Author Xavier Stéphenne Mustapha Najimi Etienne M Sokal
Format Journal Article
LanguageEnglish
Published United States Catholic University of Louvain and St Luc Clinics, Paediatric De partment (HPED), PEDI unit, Laboratory of Paediatric Hepatology and Cell Therapy, Hippocrate Avenue 10, 1200 Brussels, Belgium 07.01.2010
Baishideng
Subjects
Adult
Animals
Antioxidants - pharmacology
Cell Death
Cell Separation
Cell Survival
Cells, Cultured
Cryopreservation - methods
Cryopreservation - standards
Cryoprotective Agents - pharmacology
Hepatocytes - drug effects
Hepatocytes - metabolism
Hepatocytes - pathology
Hepatocytes - transplantation
Humans
Ice
Mitochondria, Liver - metabolism
Osmotic Pressure
Intracellular ice formation
Mitochondria
Hepatocyte
Cryopreservation
Quality
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Summary:Liver cell transplantation presents clinical benefit in patients with inborn errors of metabolism as an alternative,or at least as a bridge,to orthotopic liver transplantation.The success of such a therapeutic approach remains limited by the quality of the transplanted cells.Cryopreservation remains the best option for long-term storage of hepatocytes,providing a permanent and sufficient cell supply.However, isolated adult hepatocytes are poorly resistant to such a process,with a significant alteration both...
Bibliography:Intracellular ice formation
Mitochondria
Hepatocyte
Cryopreservation
14-1219/R
Quality
R575
Hepatocyte; Cryopreservation; Quality; Mitochondria; Intracellular ice formation
SourceType-Scholarly Journals-1
ObjectType-Commentary-2
content type line 23
ObjectType-Review-1
ObjectType-Article-4
ObjectType-Editorial-3
Correspondence to: Etienne M Sokal, MD, PhD, Professor, Catholic University of Louvain and St Luc Clinics, Paediatric Department (HPED), PEDI unit, Laboratory of Paediatric Hepatology and Cell Therapy, Hippocrate Avenue 10, B-1200 Bruxelles, Belgium. etienne.sokal@uclouvain.be
Author contributions: All authors contributed to the writing and correction of the manuscript.
Telephone: +32-2-7641387 Fax: +32-2-7648909
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v16.i1.1