Expression and clinical significance of miRNA-34a in colorectal cancer

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 15; no. 21; pp. 9265 - 9270
• Main Authors: Ma, Zhi-Bin, Kong, Xiao-Lin, Cui, Gang, Ren, Cui-Cui, Zhang, Ying-Jie, Fan, Sheng-Jin, Li, Ying-Hua
• Format: Journal Article
• Language: English
• Published: Thailand 01.01.2014
• Subjects: Adenocarcinoma - chemistry; Adenocarcinoma - genetics; Adenoma - chemistry; Adenoma - genetics; Aged; Cell Line, Tumor; Colon - chemistry; Colonic Polyps - chemistry; Colonic Polyps - genetics; Colorectal Neoplasms - chemistry; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; DNA Methylation; Female; Humans; Male; MicroRNAs - analysis; MicroRNAs - genetics; Middle Aged; Rectum - chemistry; RNA, Messenger - analysis
• ISSN: 1513-7368; 2476-762X
• DOI: 10.7314/APJCP.2014.15.21.9265

The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions. Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA- 34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a. After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05). miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.