Roles of GST-π and polβ genes in chemoresistance of esophageal carcinoma cells

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 14; no. 12; pp. 7375 - 7379
• Main Authors: Tang, Yue, Xuan, Xiao-Yan, Li, Min, Dong, Zi-Ming
• Format: Journal Article
• Language: English
• Published: Thailand 01.01.2013
• Subjects: Animals; Antineoplastic Agents - pharmacology; Blotting, Western; Cell Proliferation; Cisplatin - pharmacology; DNA Polymerase beta - antagonists & inhibitors; DNA Polymerase beta - genetics; DNA Polymerase beta - metabolism; Drug Resistance, Neoplasm - genetics; Esophageal Neoplasms - drug therapy; Esophageal Neoplasms - genetics; Esophageal Neoplasms - pathology; Flow Cytometry; Gene Expression Regulation, Neoplastic - drug effects; Glutathione S-Transferase pi - antagonists & inhibitors; Glutathione S-Transferase pi - genetics; Glutathione S-Transferase pi - metabolism; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Small Interfering - genetics; Tumor Cells, Cultured; Xenograft Model Antitumor Assays
• ISSN: 1513-7368; 2476-762X
• DOI: 10.7314/APJCP.2013.14.12.7375

The main aim of this study was to investigate the roles of GST-π and polβ genes in the chemoresistance of esophageal carcinoma cells. Eukaryotic expression vectors containing each gene were constructed and transfected into EC9706 cells, and the biological effects of the two genes assessed based on a resistance index. We additionally investigated the in vitro and in vivo anti-resistance effects of GST-π and polβ genes using recombinant lentiviruses carrying siRNAs against the two genes. Our results showed that upregulation of GST-π and polβ genes suppresses chemosensitivity of esophageal carcinoma cells to cisplatin, while downregulation of these two genes with RNAi technology reverses this chemoresistance. Multi-site injection of recombinant lentivirus targeting the GST-π gene into transplanted cDDP tumors effectively reversed their chemoresistant phenotype. However, the same treatment against the polβ gene did not lead to significant efficacy against chemoresistance.