SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

• Published in: Cellular reprogramming Vol. 22; no. 1; p. 22
• Main Authors: Zhang, Ruimen, Deng, Yanfei, Lv, Qiao, Xing, Qinghua, Pan, Yu, Liang, Jingyuan, Jiang, Mingsheng, Wei, Yingming, Shi, Deshun, Xie, Bingkun, Yang, Sufang
• Format: Journal Article
• Language: English
• Published: United States 01.02.2020
• Subjects: bovine; differentiation; skeletal muscle-derived MSCs; SQLE gene; proliferation
• ISSN: 2152-4998
• DOI: 10.1089/cell.2019.0077

In this study, Squalene epoxidase ( ) overexpression vector was transfected into bovine skeletal muscle-derived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of regulating meat quality through myogenesis. We initially profiled the expression of in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression of during cell proliferation and differentiation. Then, we constructed a bovine overexpression vector and transfected it into bovine skeletal muscle-derived MSCs by liposome transfection. Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), flow cytometry, immunofluorescence, and quantitative polymerase chain reaction assays were used to characterize cell proliferation and differentiation in detail. The results showed that the relative expression level of bovine gene in brain tissue was the highest, and in adult muscle tissue was significantly higher than that in embryonic stage. Especially, the expression of was significantly upregulated in cell differentiation stage. Furthermore, the proliferation, cell cycle, apoptosis, and myoblast differentiation assays indicated that significantly promoted the differentiation and apoptosis of bovine skeletal muscle-derived MSCs, but inhibited their proliferation. In conclusion, our study reveals the role of in myoblast differentiation. These results will provide new clues for the regulation network of bovine muscle development.