Azithromycin-resistant Neisseria gonorrhoeae spreading amongst men who have sex with men (MSM) and heterosexuals in New South Wales, Australia, 2017

• Published in: Journal of antimicrobial chemotherapy Vol. 73; no. 5; pp. 1242 - 1246
• Main Authors: Whiley, David M, Kundu, Ratan L, Jennison, Amy V, Buckley, Cameron, Limnios, Athena, Hogan, Tiffany, Enriquez, Rodney, El Nasser, Jasmin, George, C Robert, Lahra, Monica M
• Format: Journal Article
• Language: English
• Published: England 01.05.2018
• Subjects: Adolescent; Adult; Anti-Bacterial Agents - pharmacology; Azithromycin - pharmacology; Bacterial Proteins; Disease Outbreaks; Drug Resistance, Bacterial; Female; Genotype; Genotyping Techniques; Gonorrhea - epidemiology; Gonorrhea - microbiology; Heterosexuality; Homosexuality, Male; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Neisseria gonorrhoeae - drug effects; Neisseria gonorrhoeae - genetics; Neisseria gonorrhoeae - isolation & purification; New South Wales - epidemiology; Point Mutation; Repressor Proteins; RNA, Ribosomal, 23S - genetics; Transgender Persons; Young Adult; New South Wales
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dky017

To identify the genetic basis of resistance as well as to better understand the epidemiology of a recent surge in azithromycin-resistant Neisseria gonorrhoeae in New South Wales, Australia. Azithromycin-resistant N. gonorrhoeae isolates (n = 118) collected from 107 males, 10 females and 1 transsexual between January and July 2017 were genotyped using a previously described iPLEX method. The results were compared with phenotypic resistance profiles and available patient data. The iPLEX results revealed 10 different N. gonorrhoeae genotypes (designated AZI-G1 to AZI-G10) of which three were responsible for the majority of infections; AZI-G10 (74.6%, 88 isolates; 87 males and 1 transsexual), AZI-G4 (11.0%, 13 isolates; 7 males and 6 females) and AZI-G7 (6.8%, 8 isolates; 7 males and 1 female). The observed resistance was attributable to one of two different azithromycin resistance mechanisms; the 23S rRNA C2611T mutation was identified in 24% of isolates, whereas the majority of resistance (76%) was associated with a meningococcal-type mtrR variant. Additionally, one isolate was found to harbour both the 23S rRNA C2611T mutation and a type XXXIV mosaic penA sequence associated with cephalosporin resistance. These data indicate outbreaks of azithromycin-resistant gonococci amongst networks of MSM and heterosexuals in New South Wales. The results also provide further evidence that azithromycin may soon be an ineffective treatment option for gonococcal infection and highlight the urgent need to explore alternative therapies.