Comparative evaluation of plant extract effects on peritoneal, medullary and J774 cells. G8 macrophages

• Published in: Brazilian journal of biology Vol. 83; pp. e268859 - 10
• Main Authors: Marques-Santos, F, Amendoeira, M R R, Galvão, R M S, Rocha, L M, Faria, R X
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Instituto Internacional de Ecologia 2023
• Subjects: Animals; Biocompatibility; BIOLOGY; Bone marrow; Cell lineage; Cells; Chemical reduction; Composting; Drug development; Drugs; Ethics; Flowers & plants; Herbal medicine; Immunology; L-Lactate dehydrogenase; Lactate dehydrogenase; Macrophages; Mammalian cells; Mammals; Medicinal plants; Mice; Natural products; Oxidation; Oxidation-reduction potential; Peritoneum; Plant extracts; Plant Extracts - toxicity; Plant Leaves; Plant species; Plants (botany); Plants, Medicinal; Raw materials; Redox reactions; Toxicity; Toxicity testing; toxicity; natural products; macrófagos; macrophages; produtos naturais; cell death; toxicidade; extrato de planta; plant extract; morte celular
• ISSN: 1519-6984; 1678-4375; 1678-4375
• DOI: 10.1590/1519-6984.268859

The use of medicinal plants as raw material for extracts production and pure substances isolation and subsequence development of new drugs represents a constantly growing area. However, some stages are indispensable before pharmacologically evaluating natural products such as medicines. Toxicity tests in mammalian cells are essential to initiate new drugs development or verify the substance's biocompatibility. Thus, we verified the toxicity of crude extracts and fractions with different polarities obtained from the leaves and stems of eight plant species. The toxic effect was evaluated on macrophages obtained from the bone marrow and peritoneal cavity of a Swiss webster mouse and J774 macrophages. G8 cell lineage. These macrophages were cultured in a 96-well plate, and the compounds were added at a concentration of 100 µg/mL for 24 hours. After this time, the supernatant was removed. The toxicity was evaluated for lactate dehydrogenase (LDH) release assay and the resazurin assay, which uses an indicator dye to measure oxidation-reduction reactions. The results showed a difference in the percentage of toxicity when comparing the same extract in different types of macrophages. This outcome indicates that these cells from different origins may exhibit different responses when exposed to the same natural compounds.