Klf10 favors Mycobacterium tuberculosis survival by impairing IFN‐γ production and preventing macrophages reprograming to macropinocytosis
Mycobacterium tuberculosis has developed diverse mechanisms to survive inside phagocytic cells, such as macrophages. Phagocytosis is a key process in eliminating invading pathogens; thus, M. tuberculosis efficiently disrupts phagosome maturation to ensure infection. However, inflammatory cytokines p...
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Published in | Journal of leukocyte biology Vol. 112; no. 3; pp. 475 - 490 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.09.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Mycobacterium tuberculosis has developed diverse mechanisms to survive inside phagocytic cells, such as macrophages. Phagocytosis is a key process in eliminating invading pathogens; thus, M. tuberculosis efficiently disrupts phagosome maturation to ensure infection. However, inflammatory cytokines produced by macrophages in response to early M. tuberculosis infection are key to promoting bacterial clarification. IFN‐γ enhances M. tuberculosis engulfment and destruction by reprogramming macrophages from phagocytosis to macropinocytosis. Here, we show that the transcription factor Krüppel‐like factor 10 (Klf10) plays a positive role in M. tuberculosis survival and infection by negatively modulating IFN‐γ levels. Naïve Klf10‐deficient macrophages produce more IFN‐γ upon stimulation than wild‐type macrophages, thus enhancing bacterial uptake and bactericidal activity achieved by macropinocytosis. Moreover, Klf10⁻/⁻ macrophages showed cytoplasmic distribution of coronin 1 correlated with increased pseudopod count and length. In agreement with these observations, Klf10⁻/⁻ mice showed improved bacterial clearance from the lungs and increased viability. Altogether, our data indicate that Klf10 plays a critical role in M. tuberculosis survival by preventing macrophage reprogramming from phagocytosis to macropinocytosis by negatively regulating IFN‐γ production upon macrophage infection.
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By inducing Klf10 expression, Mycobacterium tuberculosis decreases INF‐γ production and impairs an INF‐γ‐mediated autocrine loop that promotes macrophages macropinocytosis, thus favoring survival and infection. |
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Bibliography: | expression Mycobacterium tuberculosis decreases IFN‐γ production and impairs an IFN‐γ‐mediated autocrine loop that promotes macrophage macropinocytosis, thus favoring survival and infection. Summary sentence Klf10 By inducing Additional supporting information can be found online in the Supporting Information section at the end of this article. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0741-5400 1938-3673 |
DOI: | 10.1002/JLB.4MA0422-288R |