Identification of a candidate CD5 homologue in the amphibian Xenopus laevis

We identified a novel T cell Ag in the South African clawed toad (Xenopus laevis) by a mAb designated 2B1. This Ag is present in relatively high levels on most thymocytes, approximately 65% of splenocytes, 55% of PBL, and 65% of intestinal lymphocytes, but is rarely seen on IgM+ B cells in any of th...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 155; no. 9; pp. 4218 - 4223
Main Authors Jurgens, JB, Gartland, LA, Du Pasquier, L, Horton, JD, Gobel, TW, Cooper, MD
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.11.1995
Subjects
Amino Acid Sequence
Animals
CD5 Antigens - genetics
CD5 Antigens - isolation & purification
Cell Differentiation
Cell Line
Freshwater
Lymphocyte Activation
Lymphocyte Cooperation - immunology
Molecular Sequence Data
Organ Specificity - immunology
Phytohemagglutinins - pharmacology
Sequence Homology, Amino Acid
Species Specificity
Spleen - immunology
T-Lymphocytes - chemistry
T-Lymphocytes - immunology
Tetradecanoylphorbol Acetate - pharmacology
Xenopus laevis
Xenopus laevis - immunology
Online AccessGet full text

Cover

More Information
Summary:We identified a novel T cell Ag in the South African clawed toad (Xenopus laevis) by a mAb designated 2B1. This Ag is present in relatively high levels on most thymocytes, approximately 65% of splenocytes, 55% of PBL, and 65% of intestinal lymphocytes, but is rarely seen on IgM+ B cells in any of these tissues. Lymphocytes bearing the 2B1 Ag proliferate in response to stimulation with Con A or PHA, whereas the 2B1- lymphocytes are reactive to LPS. Biochemical analysis indicates that this Ag is a differentially phosphorylated glycoprotein of 71 to 82 kDa. The protein core of 64 kDa bears both N- and O-linked carbohydrate side chains. The amino-terminal protein sequence of the 2B1 Ag shares significant homology with both the macrophage scavenger receptor type 1 motif and the mammalian CD5/CD6 family. The biochemical characteristics and cellular distribution of the 2B1 Ag suggest that it represents the CD5 homologue in X. laevis. While T cells constitutively express this highly conserved molecule, Xenopus B cells acquire the CD5 homologue only when they are stimulated in the presence of T cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.155.9.4218